Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETMAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGAEERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHRLTDYPDGTKVLFTIQ 3EYV Chain:H ((1-70)) --------------------------------------------------------------------EVQLVESGGGVVQPGRSLRLSCSTSGFTFSDYYMYWVRQA-PGKGLEWVAYMSNVGA-------ITDYPDTVKGRFTI-

General information: TITO was launched using: RESULT: Template: 3EYV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 168 -10253 -61.03 -146.46 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain H : 0.63 3D Compatibility (PKB) : -61.03 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.368

(partial model without unconserved sides chains): PDB file : Tito_3EYV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EYV-query.scw PDB file : Tito_Scwrl_3EYV.pdb: