Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDFMNFFILGADLPTLGGVKGWASDVVIQF--ITIVVMFIAAKNLMKLKMGGIIFVCCIGSAVTWVIKHWSEFSGWINALMEKL---------- 3L9A Chain:X ((9-88)) ---RDFFVITNSEYTFAGVH-YAKGAVLHVSPTQKRAFWVIADQENFIKQVN--------KNIEYVEKNAS--PAFLQRIVEIYQVKFEGKNVH

General information: TITO was launched using: RESULT: Template: 3L9A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 234 -56630 -242.01 -832.79 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain X : 0.65 3D Compatibility (PKB) : -242.01 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_3L9A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3L9A-query.scw PDB file : Tito_Scwrl_3L9A.pdb: