Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------MGRLVFNYRKAMREPKKIQQLTENYSLPFAV------ELIPAINY--------FIFVGLCFGFWYGVRMIFPHAFDNSYVIVIFGIPFFLTMLVTKIKPEGKNIYIYFFDFAKY----------YFFIKLPQKKYCNDRKIDLSNEKQIEFRKLV------KVVDYSNETKNAYEGNTQEFAVNKNGRRVGVLPNKKQFDSYAK--------- 1J9J Chain:A ((1-247)) MRILVTNDDGIQSKGIIVLAELLSEEHEVFVVAPDKERSATGHSITIHVPLWMKKVFISERVVAYSTTGTPADCVKLAYNVVMDKRVDLIVSGVNRGPNMGMDILHSGTVSGAMEGAMMNIPSIAISSANYESPDFEGAARFLIDFLKEFDFSLLDPFTMLNINVPAGEIKGWRFTRQSRRRWNDYFEERVSPFGEKYYWMMGEVIEDDDRDDVDYKAVREG-YVSITPIHPFLTNEQCLKKLREVYD

General information: TITO was launched using: RESULT: Template: 1J9J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 741 -66545 -89.80 -384.65 target 2D structure prediction score : 0.28 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -89.80 2D Compatibility (Sec. Struct. Predict.) : 0.28 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.150

(partial model without unconserved sides chains): PDB file : Tito_1J9J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1J9J-query.scw PDB file : Tito_Scwrl_1J9J.pdb: