Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISIMMKVSLAVFMLAGGIIKVSRVPFQVEHWRHYQYPLWFLTVTGILEIAGALAMTAGIWNRYAAIGAGVLFVVLMAGAIHAHM--FRARQSVIMAIQAMICLIVSIMIIMGSYT 3F2E Chain:A ((8-90)) FTAVAEQVSAVLSQYG---------------------------ITG-PNRAIYQGFGLKVARALNRLGGGPALVNMINGLKAYYISAFNANPTVLDAVTDIITGSPT-----GYVS

General information: TITO was launched using: RESULT: Template: 3F2E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 283 -67041 -236.89 -827.67 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -236.89 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.262

(partial model without unconserved sides chains): PDB file : Tito_3F2E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3F2E-query.scw PDB file : Tito_Scwrl_3F2E.pdb: