Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDYADYDKALYYTHR--SQW--DNLLILMVRTED--DLLSKRIEHFLHAYHFEQDYAVLEKMLYSLLRYIDHATELTYEDQIALLT 1E5P Chain:A ((86-138)) --YITSDKIFFTNKNMDRAGQETNMIVVAGKGNALTPEENEILVQFAHEKKIPVE-------------------------------

General information: TITO was launched using: RESULT: Template: 1E5P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 104 -16806 -161.59 -357.56 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -161.59 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.396

(partial model without unconserved sides chains): PDB file : Tito_1E5P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E5P-query.scw PDB file : Tito_Scwrl_1E5P.pdb: