Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAVIVDAKRT-IFGNQNGLLKPFLPEDLAAPIIRCLSRKLE---DQVDEVILGNATGRGGNLA---RLSALQAGLPLSVPGMTIDRQCGSGLEAVRYACSLIQAGAGTMYIAGGSESSSQSPFSERARFSPDAIGDPD-------MGIAAEYTAARYSISRSMQDEYALLSHQRSRNAHDEGFYREEVVALGELETDEAFLKTRPIE-------------AIIPRAKPVFDTSSGTVTAANSSGIADGAAALLVMEEEKAAALGLKPVLRFIGSAVSGIHPNFPPAAPVVAIRQLLHTHDVTPDDIDLFEINEAFAVKICVCSQELGIPFSKINVRGGALALGHPYGASGAALVTRLFYEAKRR-PDCQYAVAAIGSGGGIGLALLFEVLA 2WU9 Chain:A ((31-417)) -DVVIVAAHRTPLCKSKRGNFKDTYPDDLLAPVLRALIEKTNLNPSEVGDIVVGTVLAPGSQRASECRMAAFYAGFPETVAVRTVNRQCSSGLQAVADVAAAIKAGFYDIGIGAGLESMTTNPMAWEGSVNPAVKKFAQAQNCLLPMGVTSENVAQRFGVSRQEQDQAAVDSHRKAAAATAAGKFKDEIIPVKTKLVDPKTGDEKPITVSVDDGIRPTTTLASLGKLKPVF-KKDGTTTAGNSSQVSDGAGAVLLMKRSVAMQKGLPVLGVFRTFAAVGVDPAIMGIGPAVAIPAAVKAAGLELDDIDLFEINEAFASQFVYCRNKLGLDPEKINVNGGAMAIGHPLGATGARCVATLLHEMKRRGKDCRFGVVSMCIGTGMGAAAVFE---

General information: TITO was launched using: RESULT: Template: 2WU9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2334 -205448 -88.02 -572.28 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -88.02 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.443

(partial model without unconserved sides chains): PDB file : Tito_2WU9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WU9-query.scw PDB file : Tito_Scwrl_2WU9.pdb: