Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENQEVVLSIDAIQEPEQIKFNMSLKNQSERAIEFQFSTGQKFELVVYDSEHKERYRYSKEKM-FTQAFQNLTLESGETYDFSDVWKEVPEPGT---YEVKVTFKGRAENLKQVQAVQQFEVK 3CFU Chain:A ((105-190)) ------------KDDEERLIIEVTMENIGEDSISYNF-----IGFDLRDKNDQS-VRPVFSIEEKGRILMGGTLVSGKKVTGVLSYV--IPKGEQKHYTLVYNPFL-----------------

General information: TITO was launched using: RESULT: Template: 3CFU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 282 -25882 -91.78 -315.63 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -91.78 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_3CFU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CFU-query.scw PDB file : Tito_Scwrl_3CFU.pdb: