Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEGYLVLEDGTSFSGELDGHENCTGEAVFFTGMTGYQEVLTDPSYKGQIIVFTYPLIGNYGINEKDFESKKPQVKAAVVYEACDHFSHYEAVYSLKEYLQKWNIPLLTHVDTRAVVKKIRANGTMGATVTASKEGAEIALQPENVAEQASAQEISTFGDGNKHIALIDFG--YKKSIASSLVKRGCKVTVVPYQQ-MEAVYNIKPDGIVLSNGPG---DPKAIQPYLGKIKSIISRFPTLGICLGHQLIALAFGGNTFKLPFGHRGANHPVIDRKTKRVFMTSQNHSYVVDEQSINEEELTIRFHHVNDTSVEGLAHKKLPVMSVQFHPEAHPGPAESEWIFDDYLKNVIPARREIAHA 2YWC Chain:A ((2-120)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------VLVLDFGSQYTRLIARRLRELRAFSLILPGDAPLEEVLKHRPQALILSGGPRSVFDPDAPRP---DPRLFSSGLPLLGICYGMQLLAQELGGRV------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2YWC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 363 -59934 -165.11 -705.10 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -165.11 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.481

(partial model without unconserved sides chains): PDB file : Tito_2YWC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YWC-query.scw PDB file : Tito_Scwrl_2YWC.pdb: