Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALKAQNTISGKEGRLFLDGEEMAHIKTFEANVEKNKSEVNIMGRRMTGHKTTGANGTGTATFYKVTSKFVLLMMDYVKKGSD-PYFTLQAVLDDQSSGRGTERVTLYDVNFDSAKIASLDVDSEALEEEVPFTFEDFDVPEKLSDTF 3V4H Chain:A ((23-134)) ---------------------NEIQVLAWNWDVAQH------------------KASVSDFCFAHYIDKASPNLLSYCLLGKHIKNVQFVLRK----P-LEYLTIKFTDVIITRVDMAGSLED--RPREEIRFSFTK-----------

General information: TITO was launched using: RESULT: Template: 3V4H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 310 -34445 -111.11 -382.72 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -111.11 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.443

(partial model without unconserved sides chains): PDB file : Tito_3V4H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3V4H-query.scw PDB file : Tito_Scwrl_3V4H.pdb: