Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTRKPFIICDFDGTITMNDNIINIMKTFAPPE-WMALKDGVLSKTLSIKEGVGRMFGLLPSSLKEEITSFVLE-DAKIREGFREFVAFINEHEIPFYVISGGMDFFVYPLLEGIVEKD--RIYCNHASFD-NDYIHI---DWPHSCKGTCSNQCGCCKPSVIHELSE--PNQYIIMIGDSVTDVEAAKLSDLCFARDYL-LNECREQNLNHLPYQDFYEIRKEIENVKEVQEWLQNKNAGESSLK 1NNL Chain:A ((12-221)) --YSADAVCFDVDSTVIREEGIDELAKICGVEDAV------------PFKAALTERLALI-QPSREQVQRLIAEQPPHLTPGIRELVSRLQERNVQVFLISGGFRSIVEHVASKLNI-PATNVFANRLKFYFNGEYAGFDETQPT--------AESGGKGKVIKLLKEKFHFKKIIMIGDGATDMEACPPADAFIGFGGNVIRQQVKD-NAKWYITDFVELLG-----------------------

General information: TITO was launched using: RESULT: Template: 1NNL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 987 -138749 -140.58 -741.97 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -140.58 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.349

(partial model without unconserved sides chains): PDB file : Tito_1NNL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NNL-query.scw PDB file : Tito_Scwrl_1NNL.pdb: