Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKVIQLSDELSNKIAAGEVVERPASVVKELVENAIDADSTVIEIDIEEAGLASIRVLDNGEGMENEDCKRAFRRHATSKIKDENDLFRVRTLGFRGEALPSIASVSHLEITTSTGEG-AGTKLVL-QGGNIISESRSSSRKGTEIVVSNLFFNTPARLK-YMKTVHTELGNITDVVNRIALAHPEVSIRLRH---HGKN--LLQTNGNGDVRHVLAAIYGTAVAKKMLPL-------------HVSSLD-----FEVKGYIALPE--ITRASRNYMSSVVNGRYIKNFPLVKAVHEGYHTLLPIGRH--PITFIEITMDPILVDVNVHPSKLEVRLSKETELHDLIRDGIKDVFKQQQLIPSAQVPKKSAPAIKNEQQFITFDEKPPEKKVPEKSTAPSYSPMKLSSVVKEPVDAEEKLPPLQFDAPPIVDQEQTLEVSDVSAEQPETFEQECHEEQPQPASDRVPIMYPIGQMHGTYILAQNENGLYIIDQHAAQERIKYEYFREKVGEVEPEVQEMIVPLTFHYSTNEALIIEQHKQELESVGVFLESFGSNSYIVRCHPAWFPKGEEAELIEEIIQQVLDSKNIDIKKLREEAAIMMSCKGSIKANRHLRNDEIKALLDDLRSTSDPFTCPHGRPIIIHHSTYEMEKMFKRVM 1EA6 Chain:A ((27-363)) ---------------CSGQVVLSLSTAVKELVENSLDAGATNIDLKLKDYGVDLIEVSDNGCGVEEENFEGLTL-----------------------EALSSLCALSDVTISTCHASAKVGTRLMFDHNGKIIQKTPYPRPRGTTVSVQQLFSTLPVRHKEFQRNIKKEYAKMVQVLHAYCIISAGIRVSCTNQLGQGKRQPVVCTGGSPSIKENIGSVFGQKQLQSLIPFVQLPPSDSVCEEYGLSCSDALHNLFYISGFISQCTHGVGRSSTDRQFFFINRRPCDPAKVCRLVNEVYHMY---NRHQYPFVVLNISVDSECV-----------LLQEEKLLLAVLKTSLIGMF--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1EA6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1313 -189768 -144.53 -695.12 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -144.53 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.556

(partial model without unconserved sides chains): PDB file : Tito_1EA6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EA6-query.scw PDB file : Tito_Scwrl_1EA6.pdb: