Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAVIDDFEKLDIRTGTIVKAEEFPEARVPAIKLVIDFGTEIGIKQSSAQITKRYKPEGLINKQVIAVVNFPPRRIAGFKSEVLVLGGIPGQGDVVLLQPDQPVPNGTKIG 2NZO Chain:D ((4-113)) MAVIDDFEKLDIRTGTIVKAEEFPEARVPAIKLVIDFGTEIGIKQSSAQITKRYKPEGLINKQVIAVVNFPPRRIAGFKSEVLVLGGIPGQGDVVLLQPDQPVPNGTKIG

General information: TITO was launched using: RESULT: Template: 2NZO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 492 -42829 -87.05 -389.35 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain D : 1.00 3D Compatibility (PKB) : -87.05 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 1.00 QMean score : 0.563

(partial model without unconserved sides chains): PDB file : Tito_2NZO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2NZO-query.scw PDB file : Tito_Scwrl_2NZO.pdb: