Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNIGEIISNFEGIIGALLGVIVTLILTHILKHFGQIKFYIVDFEIYFKTDNDGWGTNVMPSKDEAKQIEIHSQIEIYNGAEIPKVLREIKFCFYKNTNLIVSVTPDDKATTEEFAEFGYYRDKLFNINLPSKQIIAINIIKFLNEKETKQVKKCNRVYLEAKDHNGKMYKVFLGEF 3O3N Chain:B ((154-188)) ----------------------------------------------------------------EIEEAKIHESIEVYN--EHRKTMRDFVEVAYKHSNTI----------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3O3N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 11 -1888 -171.64 -53.94 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain B : 0.54 3D Compatibility (PKB) : -171.64 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.456

(partial model without unconserved sides chains): PDB file : Tito_3O3N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3O3N-query.scw PDB file : Tito_Scwrl_3O3N.pdb: