Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MTQLLIIFAAAAAGLFFFEDVRDVLKLWDIRDMRIIWYGVSIAVIVILADMAVMKWFPSHL---YDDGGINKK----IFSKRSIPHIIFLTLLIAFAEEM--------LFRGVLQTHIGLWTASLIFAALHF-RYLSKWLLFIMVTAISFLLGLMYEWTGNLFVPMTAHFIIDAVFACQIRFEHVRRDKHDEHVESREKKSPESL----------------------------- 4LFU Chain:A ((5-240)) DFFSWRRTMLLRFQRMETAEEVYHEIELQAQQLE---YDYYSLCVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSEAQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRESLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNMQKKINAPNKTQVACYAAATGLI

General information: TITO was launched using: RESULT: Template: 4LFU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 768 -53805 -70.06 -289.27 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -70.06 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.216

(partial model without unconserved sides chains): PDB file : Tito_4LFU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LFU-query.scw PDB file : Tito_Scwrl_4LFU.pdb: