Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMDFLSKTPEPPYYAVIFSSVKS-ENDTGYGETAERMVSLAADQPGFLGVESVREA-D-GRGITVSYWDSMDAINHWRHHTEHQAAKEKGRSVWYESYAVRVAKVDRQRLFQENTND 3E8O Chain:A ((18-113)) -----------TMVISHGTLSASAEHAAHLRQLLVHIAQATRQEDGCLLYLVSEDLSQPGHFLITEHWDNLGAMHTHLALPGVTQAIDALKHLNVTDLKITAYEAGE----------

General information: TITO was launched using: RESULT: Template: 3E8O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 346 -18793 -54.32 -202.08 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -54.32 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_3E8O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3E8O-query.scw PDB file : Tito_Scwrl_3E8O.pdb: