Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTATIIDGKETAREKREQLAKEVEELKKQ--GVTPGLAVILIGDDPASHSYVRGKKKAAETMGM-NFKLDQFDSSLTEAELLSIIDQYNQDPEFHGILVQLPLPDHISEKAVIERISPDKDVDGFHPLNVGKMLLGED--TFLPCTPHGIVELLKKTNIDLSGKEVVVVGRSNIVGKPVGQLLLNENATVTYCHSRTE--NITEHTKKADILVVAVGRANFISADQIKEGAVVIDVGV------NRLENGKLCGDVEFEGAKEKASFITPVPGGVGPMTITMLAHNTVKSAKRTLS 4CJX Chain:A ((25-316)) --AVVIDGRAVAKAIQKELTEEVALLERRYKGRRPGLSTIICGKRKDSQTYVRLKRKAAAACGFRNFSV-ELPANVTQEALEREVIRLNEEEACHSIVVQLPLPPHIDKVAALSKIKPEKDADCLLPVNVGQLHIRERNPAIVPCTASAVMELLRCSGVEICGKRVVVLGRGDIAGLPVATLLANEDATVTVVHSATPLCDIADIVRASDIVVSAAGQPGLVRGEWIKLGAAVIDVGTTPVADPSKVPGYRLVGDVCFDVARKRAAYITPVPGGVGPVTVSMLLKNTLTMFKRSV-

General information: TITO was launched using: RESULT: Template: 4CJX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1487 -21825 -14.68 -78.23 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -14.68 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.672

(partial model without unconserved sides chains): PDB file : Tito_4CJX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CJX-query.scw PDB file : Tito_Scwrl_4CJX.pdb: