Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYDTIVIGAGQAGISIGYYLKQSDQKFIILDKSHEVGESWKDRYDSLVLFTSRMYSSLPGMHLEGEKHGFPSKNEIVAYLKKYVKKFEIPIQLRTEVISVLKIKNYFLI-KTNREEYQTKNLVIATGPFH-TPNIPSISKDLSDNINQLHSSQYKNSKQLAYGNVLVVGGGNSGAQIAVELSK-ERVTYLACSNKLVYFPLMIGKRSIFWWFDKLGVLHASHTSIVGKFIQKKGDPVFGHELKHAIKQKEIILK--KRVIAAKQN-----EIIFKD--S--STLEVNNIIWATGFRNPLCWINIKGVLDQEGRIIHH-RGVSPVEGLYFIGLPWQHKRGSALLQGVGNDAEYIVKQMNGE 2ZBW Chain:A ((5-313)) HTDVLIVGAGPTGLFAGFYVGMRGLSFRFVDPLPEPGGQLTALYPEKYIY------DVAGFPK-------VYAKDLVKGLVEQVAPFNPVYSLGERAETLEREGDLFKVTTSQGNAYTAKAVIIAAGVGAFEPRRIGAPGEREFEGRGVYYA-VKSKAEFQGKRVLIVGGGDSAVDWALNLLDTARRITLIHRRP-QFRAHE---A-SVKEL------------------------------MKAHEEGRLEVLTPYELRRVEGDERVRWAVVFHNQTQEELALEVDAVLILAGYITKLGPLANWGLALEKNKIKVDTTMATSIPGVYACGDIVTYPGKLPLIVLGFGEAAIAANHAA--

General information: TITO was launched using: RESULT: Template: 2ZBW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1591 -55114 -34.64 -187.46 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -34.64 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.460

(partial model without unconserved sides chains): PDB file : Tito_2ZBW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZBW-query.scw PDB file : Tito_Scwrl_2ZBW.pdb: