Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYAIIKTGGKQIKVEEGQTVYIEKLAAEAGETVTFEDVLFVG-GDNVKVGNPTVEGATVTAKVEKQGRAKKITVFRYKPKKNVHKKQGHRQPYTKVTIEKINA 3J8G Chain:R ((1-103)) MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANGEEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGISA

General information: TITO was launched using: RESULT: Template: 3J8G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain R - contact count / total energy / energy per contact / energy per residue : 412 2752 6.68 26.98 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain R : 0.88 3D Compatibility (PKB) : 6.68 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.306

(partial model without unconserved sides chains): PDB file : Tito_3J8G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3J8G-query.scw PDB file : Tito_Scwrl_3J8G.pdb: