Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDTAMARLNEGTIVIYALSVLFYFIDFLQHNRKAGKMAFWLLSIVWTLQTVYLAYFMWVTGRFPVLNVTEALYFYAWVLVTLSLVLTKLLRVDFIVFFTNVIGFSMIAIHTFSPTEQQSAAFSGQLVSELLVIHITMAILSYGAFSLSFVFSVLYMFQYHLLKKKKWGKWLLRIEDLSKLDYMAYVLNVIGVPMLLLSLILGVIWAYVSLETLYWFDAKVLGSFVVLLLYSYYLYIRLIKELQGKVAALWNTACFLVLMINYFLLGSLSQFHWFS 2BE6 Chain:D ((11-28)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FYATFLIQEYFRKFKKRK-----------------------------

General information: TITO was launched using: RESULT: Template: 2BE6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2 -39 -19.25 -2.14 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain D : 0.34 3D Compatibility (PKB) : -19.25 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.34 QMean score : 0.378

(partial model without unconserved sides chains): PDB file : Tito_2BE6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BE6-query.scw PDB file : Tito_Scwrl_2BE6.pdb: