Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIDSWLNDRLDIAKEAGVHRNLRSMNGAPVPERNIDGENQTVWSSNNYLGLASDRRLIDAAQTALQQFGTGSSGSRLTTGNSVWHEKLEKKIASFKRTEAALLFSSGYLANVGVLSSLPEKEDVILSDQLNHASIIDGCRLSKADTVVYRHIDMNDLENKLNETQRYQRRFIVTDGVFSMDGTIVPLDQIISLAKRYHAFVVVDDAHATGVLGDSGRGTSEYFGVYP--DIVIGTLSKAVGTEGGFAAGSAVFIDFLLNHARTFIFQTAIPPASCA----------AAHE--------AFNIRTSLKNMGYVVKGD--HTPIIPVVIGDAHKTVIFAEKLQGKGIYAPAIRPPTVAPGESRIRITITSDHSMGDIDDLLKTFHSIGKELHII 2W8U Chain:A ((59-419)) --------------------------------EAVIRGKDTILLGTYNYMGMTFDPDVIAAGKEALEKFGSGTYGSRMLNGTFHDHMEVEQALRDFYGTTGAIVFSTGYMANLGIISTLAGKGEYVILDADSHASIYDGCQQGNAEIVRFRHNSVEDLDKRLGRLPKEPAKLVVLEGVYSMLGDIAPLKEMVAVAKKHGAMVLVDEAHSMGFFGPNGRGVYEAQGLEGQIDFVVGTFSKSVGTVGGFVVSNHPKFEAVRLACRPYIFTASLPPSVVATATTSIRKLMTAHEKRERLWSNARALHGGLKAMGFRLGTETCDSAIVAVMLEDQEQAAMMWQALLDGGLYVNMARPPATPAGTFLLRCSICAEHTPAQIQTVLGMFQAAGRAVGVI

General information: TITO was launched using: RESULT: Template: 2W8U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1956 -130839 -66.89 -385.95 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -66.89 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.484

(partial model without unconserved sides chains): PDB file : Tito_2W8U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W8U-query.scw PDB file : Tito_Scwrl_2W8U.pdb: