Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPSVESFELDHNAVVAPYVRHCGVHKVGTDG-VVNKFDIRFCQPNKQAMKPDTIHTLEHLLAFTIRSHAEKYDHFDIIDISPMGCQTGYYLVVSGEPTSAEIVDLLEDTMKEAVE-IT--EIPAANEKQCGQAKLHDLEGAKRLMRFWLSQDKEELLKVFG 4XCH Chain:D ((46-182)) --------LDHTIVKAPYIRLIS-EEVGPKGDIITNFDIRLIQPNENAMDTAGLHTIEHLLAKLIRQRID-----GLIDCSPFGCRTGFHMIMWGKQDSEKIAQVIKSSLEEIAEGITWEDVPGTTIESCGNYKDHSLHSAKEWAKLILSQ----------

General information: TITO was launched using: RESULT: Template: 4XCH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 563 -6835 -12.14 -51.39 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain D : 0.80 3D Compatibility (PKB) : -12.14 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.617

(partial model without unconserved sides chains): PDB file : Tito_4XCH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XCH-query.scw PDB file : Tito_Scwrl_4XCH.pdb: