Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKKDEHLRKPEWLKIKLNTNENYTGLKKLMRENNLHTVCEEAKCPNIHECWAVRRTATFMILGSVCTRACRFCAVKTGLP---------TELDLQEPERVADSVALMNLKHAVITAVARDDQKDGGAGIFAETVRAIRRKSPFTTIEVLPSDMGGNYDNLKTLMDTRPDILNHNIETV-RRLTPRVRA-RATYDRSLEFLRRAKEMQPDIPTKSSIMIGLGETKEEIIEVMDDLLANNVDIMAIGQYLQPTKKHLKVQKYYHPDEFAELKEIAMQKGFSHCEAGPLVRSSYHADEQVNEASKKRQAQA 1TV8 Chain:A ((16-191)) ---------------------------------------------------------LRLS-VTDRCNFRCDYCMPKEVFGDDFVFLPKNELLTFDEMARIAKVYAELGVKKIRITGGE-PLM----RRDLDVLIAKLNQIDGIEDIGLTTNGLLLKKH-GQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSI--GLNVKVNVVIQKGINDDQIIPMLEYFKDKHIEI-------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1TV8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 744 11097 14.92 67.25 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 14.92 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.346

(partial model without unconserved sides chains): PDB file : Tito_1TV8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TV8-query.scw PDB file : Tito_Scwrl_1TV8.pdb: