Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKELQEHELDHIEDDVYLLYLYTPFCGTCQLASKMLTVVKEMLPDVAFYQNNVNYSPTFAKAYQIESVPCFLLFKGGKMVERGYAFHSVSYLYELIKQKSSSASHL 2WZ9 Chain:A ((35-122)) -----------------VVHFWAPWAPQCAQMNEVMAELAKELPQVSFVKLEAEGVPEVSEKYEISSVPTFLFFKNSQKIDRLDGAHA-PELTKKVQRHASSGSFL

General information: TITO was launched using: RESULT: Template: 2WZ9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 340 -67203 -197.65 -763.66 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -197.65 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.598

(partial model without unconserved sides chains): PDB file : Tito_2WZ9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WZ9-query.scw PDB file : Tito_Scwrl_2WZ9.pdb: