Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRYMKLFFLVTDIGFILYWLSAGFSLIPESWAFKHHDHPFMIAWNWSFFPLDILISVTGLYSLYLQRVNRADWKLMALISLVLTCCSGLQALSFWTFSSDFDLVWWLFNGYLLIYPLFFIHLLLKEGRRTKQS 1WE6 Chain:A ((1-111)) ----------GSSGSSGKFDESA--LVPEDQFLAQHPGPATIRVSKPNENDG------QFMEITVQSLSENVGSLKEKIAGEIQIPANKQKLSGKAGFLKDNMSLAHYN----VGAGEILTLSLRERSGPSSG

General information: TITO was launched using: RESULT: Template: 1WE6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 437 -67288 -153.98 -606.20 target 2D structure prediction score : 0.35 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -153.98 2D Compatibility (Sec. Struct. Predict.) : 0.35 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.150

(partial model without unconserved sides chains): PDB file : Tito_1WE6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WE6-query.scw PDB file : Tito_Scwrl_1WE6.pdb: