Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREEEKKTSQVKKLQQFFRKRWVFPAIYLVSAAVILTAVLWYQSVSNDEVKDQLADNGGNSAYDNNDDAVEVGKSMENVAMPVVDSENVSVVKKFYETDAAKEEKEAALVTYNNTYSLSKGIDLAEKDGKDFDVSASLSGTVVKAEKDPVLGYVVEVEHADGLSTVYQSLSEVSVEQGDKVKQNQVIGKSGKNLYSEDSGNHVHFEIRKDGVAMNPLNFMDKPVSSIEKAATQETEESIQQSSEKKDGSTEKGTEEKSGEKKDDSTDKSGSKESSTTEDTEQS 3SLU Chain:B ((241-340)) -----------------------------------------------------------------------------------------------------------------HTWRLHTGIDYAAPQGTP--VRASADGVITFKGRKGGYGNAVMIRHANGVETLYAHLSAFSQAQGN-VRGGEVIGFVGST--GRSTGPHLHYEARINGQPVNPVS-----------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3SLU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 459 -16749 -36.49 -167.49 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -36.49 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.347

(partial model without unconserved sides chains): PDB file : Tito_3SLU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SLU-query.scw PDB file : Tito_Scwrl_3SLU.pdb: