Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKKQVSHAIIISVMLSFVIAVFHTIHASELTPLAQMAEGMERQDVSIDKWTLHAKQNLSLTEKEFYQKVQRLKQEYRQYDWVIAREDKMIKAIGTYTDKKNRTSFRLQLVTTLKKHNPTSYLLYEQMSLETPDSWNDTYEQFERETLGIFQEKVVIFTCLNGHLDDNMNIVLQKKANQLLNEFQARSVEHVVEPNFVSISAFTDEWEEYIMTSKHKMNLQIALRSAGMGGKHTVTVGTPIVTTEY 1EH1 Chain:A ((61-110)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PDPRTLVVQSWDQNALKAIEKAIRDSDLGLNPSN----KGDALYINIPPLTEER

General information: TITO was launched using: RESULT: Template: 1EH1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 119 -14016 -117.78 -280.32 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -117.78 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.357

(partial model without unconserved sides chains): PDB file : Tito_1EH1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EH1-query.scw PDB file : Tito_Scwrl_1EH1.pdb: