Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVQKRNHFLPYSLPLIGKEEIQEVTETLESGWLSKG-PKVQQFEKEFAAFVGAKHAVAVNSCTAALFLALKAKGIGPGDEVITSPLTFSSTANTIIHTGATPVFADIDENTLNIDPVKLEAAVTPRTKAVVPVHFGGQSCDMDAILAVAQNHGLFVLEDAAHAVYTTYKQRMIGSIGDATAFSFYATKNLATGEGGMLTTDDEELADKIRVLSLHGMSKAAWNRYSSNGSWYYEVESPGYKMNMFDLQAALGLHQLKRLDDMQKRREEIAGRYQTAFQQIPGLITPFVHDDGRHAW--HLYVL-QVDEKKAGVTRSEMITALKDEYNIGTSVHFIP-VH-IHPYYQKQFGYKEADFPNAMNYYKRTLSLPLYPSMSDDDVDDVIEAVRDIVKGAD 2PO3 Chain:A ((36-380)) ---------------------ERLDRALDSQWLSNGGPLVREFEERVAGLAGVRHAVATCNATAGLQLLAHAAGLT--GEVIMPSMTFAATPHALRWIGLTPVFADIDPDTGNLDPDQVAAAVTPRTSAVVGVHLWGRPCAADQLRKVADEHGLRLYFDAAHALGCAVDGRPAGSLGDAEVFSFHATKAVNAFEGGAVVTDDADLAARIRALHNFGFDL-------PGGS-----PAGGTNAKMSEAAAAMGLTSLDAFPEVIDRNRRNHAAYREHLADLPGVL---VADHDRHGLNNHQYVIVEIDEATTGIHRDLVMEVLKAE-GVHTRAYFSPGCHELEPYR----GQPHAPLPHTERLAARVLSLPTGTAIGDDDIRRVADLLR-------

General information: TITO was launched using: RESULT: Template: 2PO3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2014 -40307 -20.01 -118.90 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -20.01 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.552

(partial model without unconserved sides chains): PDB file : Tito_2PO3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PO3-query.scw PDB file : Tito_Scwrl_2PO3.pdb: