Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPKVSVIMTSYNKSDYVAKSISSILSQTFSDFELFIMDDNSNEETLNVIRPFLNDNRVRFYQSD---------ISGVKERTEKTRYAALINQAIEMAEGEYITYATDDNIYMPDRLLKMVRELDTHPEKAVIYSASKTYHLNENRDI--VKETVRPAAQVTWNAPCAIDHCSVMHRYSVLEKVKEKFGSYWDESPAFYRIGDARFFWRVNHFYPFYPLDEELDLNYITDQSIHFQLFELEKNEFVRNLPPQRNCRELRESLKKLGMG 1QG8 Chain:A ((1-255)) -PKVSVIMTSYNKSDYVAKSISSILSQTFSDFELFIMDDNSNEETLNVIRPFLNDNRVRFYQSDISGVKERTEKTR-------Y-AAL-INQAIEMAEGEYITYATDDNIYMPDRLLKMVRELDTHPEKAVIYSAS--KTYHLN---DIVKETVRPAAQVTWNAPCAIDHCSVMHRYSVLEKVKEKFGSYWDESPAFYRIGDARFFWRVNHFYPFYPLDEELDLNYIT--------------EFVRNLPPQRNCRELRESLKKLGMG

General information: TITO was launched using: RESULT: Template: 1QG8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1186 -10743 -9.06 -47.32 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.92 3D Compatibility (PKB) : -9.06 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.92 QMean score : 0.595

(partial model without unconserved sides chains): PDB file : Tito_1QG8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QG8-query.scw PDB file : Tito_Scwrl_1QG8.pdb: