Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNVPFWTEEHVRATLPERDAESHKGTYGTALLLAGSDDMPGAALLAGLGAMRSGLG--KLVIGTSENVIP-------LIVPVLPEATYWRDGWKKAADAQLEETYRAIAIGPGLPQTESVQQAVDHVL-TADCPVILDAGA---LAKRTYPKREGPVILTPHPGEFFRMTGVPVNELQKKRAEYAKEWAAQLQTVIVLKGNQTVIAFPDGD-CWLNPTGNGALAKGGTGDTLTGMILGMLCCHEDPKHAVLNAVYLHGACAELWTDEHSAHTLLAHELSDILPRVWKRFE 3RSS Chain:A ((218-501)) INRYVITREMVRSLLPERPRDSHKGTYGKVLIIAGSRLYSGAPVLSGMGSLKVGTGLVKLAVPFPQNLIATSRFPELISVPIDTEKGFFSLQNLQEC-LELSKDVDVVAIGPGLGNNEHVREFVNEFLKTLEKPAVIDADAINVLDTSVLKERKSPAVLTPHPGEMARLVKKTVGDV-KYNYELAEEFAKENDCVLVLKSATTIVT--DGEKTLFNITGNTGLSKGGSGDVLTGMIAGFIAQGLSPLEASTVSVYLHGFAAELF--EQDERGLTASELLRLIPEAIRRLK

General information: TITO was launched using: RESULT: Template: 3RSS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1685 -6310 -3.74 -23.37 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -3.74 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_3RSS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RSS-query.scw PDB file : Tito_Scwrl_3RSS.pdb: