Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKVHQSACPLNCWDSCGFLVTVD---DGKV-TKVDGDPNHPITEGKICGRGRMLETKTNSPDRLRYPMKK---QNGE--FVRISWEQALDEIADKLREIKETSETTAVLHSHDYANNGLLKALDQRFFNGYGGVTEIVGSICWGSGIEAQSWDFGRSYG-----HGPLDIY---NSKHVVVWGRNVSRTN-MHLYHHLQQVKKKGATITVIDPIFNPTAKLADRYISVKPGMDGWLAAAVLKVLIEMGRTDETFISEHSVGFDDVKELLKTVSLEEFIVKTETSMEELEYLAGLYA-DGPVSTFMGLGMQRYKNGGGTIRWIDALVAASGNV--GIKGGGANFGN------VQIGESFAKTKLTLPELKTTSRSF--------------------------SMMTQAEEVLTAADP----AIEMIIVTCGNPLTQVPNTNKVRQAFEKVPMTVAIDSIMTDTAELCDYVLPTATVFEEEDIYYSSMYHHYVQYGKKLVEPQGEAKSDSWIWSELAKRLGFGELFEYSTQ-EFLEMGLSSLEAEDVTLERLKEKGHLP-LPVKQVPWDDYQFLTPSGKFEFTSSLAEQKGFSGSLQLNVPEESVFHNEELAGKYPYTLLSIHPQRSNHSQ--HVPFIEKLQHVQVD----ISPDIAAGQDLQDGDEVVIFNDRGSMKGKVKVMKQAHAKTINIDEGMWAAFGGSVNALTNDTNSDNGMGSTLFDCLVGLKKA 2E7Z Chain:A ((8-677)) -------SCDINC------VVEAEVKADGKIQTKSISEPHPTTPPNSICMKSVNADTIRTHKDRVLYPLKNVGSKRGEQRWERISWDQALDEIAEKLKKIIAKYGPESLGVSQTEINQQSEYGTLRRFMN-------LLGSPNWTS---AMYMCIGNTAGVHRVTHGSYSFASFADSNCLLFIGKNLSNHNWVSQFNDLKAALKRGCKLIVLDPRRTKVAEMADIWLPLRYGTDAALFLGMINVIINEQLYDKEFVENWCVGFEELKERVQEYPLDKVAEITGCDAGEIRKAAVMFATESPASIPWAVSTDMQKNSCSAIRAQCILRAIVGSFVNGAEILGAPHSDLVPISKIQMHEALPEEKKKL-QLGTETYPFLTYTGMSALEEPSERVYGVKYFHNMGAFMANPTALFTAMATEKPYPVKAFFALASNALMGYANQQNALKGLMNQDLVVCYDQFMTPTAQLADYVLPGDHWLERPVVQPNWEGIPFGNTSQQVVEPAGEAKDEYYFIRELAVRMGLEEHFPWKDRLELINYRISPTGME--WEEYQKQYTYMSKLPDYFGP-EGVGVATPSGKVELYSSVFEKLGYDPLPYYHEPLQTEISDPELAKEYPLILFAGLREDSNFQSCYHQPGI--LRDAEPDPVALLHPKTAQSLGLPSGEWIWVETTHGRLKLLLKHDGAQPEGTIRIPHGRWC---------------------------------

General information: TITO was launched using: RESULT: Template: 2E7Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3744 110788 29.59 183.12 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 29.59 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_2E7Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2E7Z-query.scw PDB file : Tito_Scwrl_2E7Z.pdb: