Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDSKLIFFDIDGTIYDHDKNIPESTRKTVAELQRQGHHVFIASGRSPFLVKPILEELG-IHSFISYNGQFVVFENQ-VIYKNPLPENAIRRLLKQADEGKHPVVFMAEDTMKATVADH-PHV-LEGIGS-L------KT-DYP---ETDDLFYEG--KEIFQLLLFCQDEEEKAYA---AF---PEFDLVRWHELSTDVLPHGGSKAEGIKKVIERLPFDIGDTYAFGDGLNDLQMIEYVGTGVAMGNAVPELKEIADFVTKPVDEDGIAYAVKELGLLK 3DNP Chain:A ((6-275)) --KQLLALNIDGALLRSNGKIHQATKDAIEYVKKKGIYVTLVTNRHFRSAQKIAKSLKLDAKLITHSGAYIAEKIDAPFFEKRISDDHTFNIVQVLESYQCNIRLLHE----KYSIGNKKKVNSNLLGKALIHPSDPIFYPVQFVESLSD-LLMDEPVSAPVIEVYTE-HDIQHDITETITKAFPAVDVIRVNDEKLNIVPKGVSKEAGLALVASELGLSMDDVVAIGHQYDDLPMIELAGLGVAMGNAVPEIKRKADWVTRSNDEQGVAYMMKEYFR--

General information: TITO was launched using: RESULT: Template: 3DNP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1449 -147041 -101.48 -595.31 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -101.48 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.556

(partial model without unconserved sides chains): PDB file : Tito_3DNP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DNP-query.scw PDB file : Tito_Scwrl_3DNP.pdb: