Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIEIGQKAPDLELKGDHGETVKLSDYKGKYIVLYFYPKDMTPGCTTEACDFRDSHESFAELDAVIIGVSPDSQEKHGKFKEKHNLPFLLLVDDEHKLAEAFDVWKLKKNFGKEYMGIE-RSTFLIDKEGRLIKEWRKVKVKDHVAEALQTLKDMSEK 5ENU Chain:A ((1-152)) MSVEVDRQVPDFTAPAT-GGDISLSDLKGRKLVLYFYPKDNTPGCTTEGLQFRELYPKFKKAGAEIIGVSRDSLRSHDNFKAKLELPFPLISDADEALCALFDVIKMKKMYGK-EVRGIERSTFLIDADGVLRQAWRGIKVPGHVDDVLSAVQA----

General information: TITO was launched using: RESULT: Template: 5ENU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 717 -81574 -113.77 -540.23 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -113.77 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_5ENU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ENU-query.scw PDB file : Tito_Scwrl_5ENU.pdb: