Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence METIIIALIAFIIGIIAIPIVLFAWIYIKDEKQQEHSILRNYPVIGRFRYILEKIGPELRQYLYSNDNEEQPFSRKEYEQTVISGKYKSRMMGFGSVRDFDKPGYYIRNAMFPKQREEMHVNQTPKIETQIYKMDADNLFKRKEHAEHIKADPYFLHPDDVQVIGEHTCEKPFYVKGLVGQSAMSYG--SLGERAVTALSKGLHLAGGTWMNTGEGGLSE--YHLKGGADIICQIG-PGLFGVRKRNGEFSWEEFKRKSRIDQIKAFELKLAQGAKTRGGHVDGAKVSEEVADIRNVEPGKSIDSPNRFYEFSNPPEMLDFIEKLRDVGQKPVGIKLVAGHPEELHELFSHMQKSGKHPDFITIDGSEGGTGASFYE----LADTVGLPIMTALPIVDTLLKQYGLRSQLKIFASGKLLTPDKIAVALALGADFVNIARGMMFSVGCIRALVCHTNTCPAGVATTDPKLQKALSVEEKQHRVCNYVISLREGLFNLAAAAGINSPIHFSKEHVVYRKEDGSIINIDNLIHQFVS 1P0K Chain:A ((40-335)) ----------------------------------------------------------------------------------------------------------------------------------------------------------LEQVDISTKIGEL------SSSSPIFINAMTGGGGKLTYEINKSLARAASQA-GIPLAVGSQMSALKDPSERLSYEIVRKENPNGLIFANLGS-EATAAQAKEAVEMIGANALQIHLNVIQ---------EI----------------------------FSGALKRIEQICSRVSVPVIVKEVGFG--MSKASAGKLY--EAGAAAVDIGG-----------RQISFFNSWGISTAASLAEIRSEF------PASTMIASGGLQDALDVAKAIALGASCTGMAGHFLKALT-----------------------------DSGEEGLLEEIQLILEELKLIMTVLGARTIADLQKAPLVIKGETHHW------------

General information: TITO was launched using: RESULT: Template: 1P0K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1486 -150961 -101.59 -571.82 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -101.59 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.435

(partial model without unconserved sides chains): PDB file : Tito_1P0K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1P0K-query.scw PDB file : Tito_Scwrl_1P0K.pdb: