Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRRVETITFDGGTLEYSVTGKGTPILVMHGGHS--NCYEEFGYT-ALIEQGYSIITPSRPGYGRTSK--EIGKSLANACRFYVKLLDHLQIESVHVIAISAGGPSGICFASHYPERVNTLTLQSAVTKEWLTPKDTEYKLGEILFRPPVEKWIWKLISSLNNAFPRLMFRAMSPQFSTLPFQRIK--SLMNEKDIEAFRKM--NSRQRSGEGFLIDLSQTAAVSLKDLQAIICPVLIMQSVYDGLVDLSHAHHAKEHIRGAVLCLLHSWGHLIWLGKEAAETGSILLGFLES 1IUN Chain:A ((8-252)) -----KSILAAGVLTNYHDVGEGQPVILIHGSGPGVSAYANWRLTIPALSKFYRVIAPDMVGFGFTDRPENYNYSKDSWVDHIIGIMDALEIEKAHIVGNAFGGGLAIATALRYSERVDRMVLMGAAG--------TRFDVTEGL------NAVWGYTPSIENMRNLLDIFAYDRSLVTDELARLRYEASIQPGFQESFSSMFPEPRQR-----WID---ALASSDEDIKTLPNETLIIHGREDQVVPLSSSLRLGELIDRAQLHVFGRCGH---------------------

General information: TITO was launched using: RESULT: Template: 1IUN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1328 -168240 -126.69 -712.88 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -126.69 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.414

(partial model without unconserved sides chains): PDB file : Tito_1IUN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1IUN-query.scw PDB file : Tito_Scwrl_1IUN.pdb: