Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTSNRRMGLFYVITGATCWGIGGTVAKKLFQEYQIDVNWLVTTRLLTAGMLLLMLQLFRKDRSQVIEVWKHKASAGQLLIFGLLGMLAVQYTYMASIQHGNAAVATLLQYLSPVIVIIYTLLRKQTVLTKQDIITVVLALVGCFFLLTNGSMSQLSVPAAAVVWGVLSGFAAAFYTLYAVGLLNKFDSLVVVGWAMIIGGFALGFIHPPWQLDFQRLTAEAYAYILFVILFGTMIAFWFFIKSLESLSPKETSLLGSLEPLSAVVTTVVWLKAPFGSFQWIGAICIIGITLILALHKEPSMESEKSILKIRNHANRDI 4RVY Chain:T ((1-30)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------METITYVFIFACIIALFFFAIFFREPPRIT-------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4RVY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain T - contact count / total energy / energy per contact / energy per residue : 3 -1224 -407.83 -40.78 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain T : 0.42 3D Compatibility (PKB) : -407.83 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.42 QMean score : 0.179

(partial model without unconserved sides chains): PDB file : Tito_4RVY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RVY-query.scw PDB file : Tito_Scwrl_4RVY.pdb: