Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPSTVINLYYLKINSISGNGSITIGEAAYNSPTNNQKSQGTNSSFGDTSPTESVM----------ENFLNDPDVNDQTSIG--NSDTSNVNAPPIAPPPILD- 2K13 Chain:X ((1-103)) EEREDCWTFYANRKYTDFDKSFKKSSDLDECKKTCFKTEYCYIVFEDTVNKECYYNVVDGEELDQEKFVVDENFTENYLTDCEGKDAGNAAGTGDESDEVDED

General information: TITO was launched using: RESULT: Template: 2K13.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 355 8163 22.99 90.70 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain X : 0.67 3D Compatibility (PKB) : 22.99 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.056

(partial model without unconserved sides chains): PDB file : Tito_2K13.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2K13-query.scw PDB file : Tito_Scwrl_2K13.pdb: