Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MKAWKVEPYEISKAMELIYK---YLLLDKKDFSLEEF------YKLTIYAIKWKLEQAQFPLYLESTKKSH----QNVIPQPFKIKGNVLYKTVIKNSGDFEE 1QQG Chain:A ((159-262)) AFKEVWQVILKPKGLGQTKNLIGIYRLCLTSKTISFVKLNSEAAAVVLQLMNIR-RCGHSENFFFIEVGRSAVTGPGEFWMQVDDSVVAQNMHETILEAMRAMSD

General information: TITO was launched using: RESULT: Template: 1QQG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 385 -54609 -141.84 -613.58 target 2D structure prediction score : 0.20 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -141.84 2D Compatibility (Sec. Struct. Predict.) : 0.20 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.119

(partial model without unconserved sides chains): PDB file : Tito_1QQG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QQG-query.scw PDB file : Tito_Scwrl_1QQG.pdb: