Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIIRKISHFAGQTFGIWVIVFAVLGFSFPSL-----FTWISSYITIFLGIIMFGMGLTLQADDFKELVRKPWQVIIGVIAQYTIMPLV--AFGLAFGLHL--PAEIAVGVILVGCCPGGTASNVMTFLA-K-GNTALSVAVTTISTLLAPVVTPLLIMLFAKEWLPVSPGSLFISILQAVLFPIIAGLIVKMFFRKQVAKAVHALPLVSVIGIVAIVSAVVSGNRENLLQSGLLIFSVVILHNGIGYLLGFLCAKLLKMDYPSQKAIAIEVGMQNSGLGAALATAHFSPLSAVPSAIFSVWHNLSGSMLATYWSKKVKKKQAGSKSSNLSL 4CZ8 Chain:A ((34-174)) ----------------IFILTGLVIGPLLKLIPRDLAHEIFDFVRVFGLVIILFTEGHNLSWRLLKKNMPT---IVTLDTIGL-ILTALIAGFIFKVVFNSSFLLGFLFGAIIGATDPATLIP-LFRQYRVKQDIETVIVTESIFNDPLGIVLTLIAISMLV--------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4CZ8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 316 -66037 -208.98 -507.97 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : -208.98 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.136

(partial model without unconserved sides chains): PDB file : Tito_4CZ8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CZ8-query.scw PDB file : Tito_Scwrl_4CZ8.pdb: