Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELIIILLALGLLMFTAYRGFSVILFAPICALFAVLLTDPSHVLPFFSSIFMEKMAGFIKLYFPVFLLGAIFGKVVEMAGLAAS-IAKTIVRL-VGA-KR--AILAIVLMGAVLTYSGVSLFVVVFAVYPFAKNMFQEANIPKRLIPGTIALGAFTFTMDALPGTPQIQNVIPTSFFKTDIYAAPWLGLMGAVIVLAAGMLYLESRRKKAQASGEGYGGFDSQNAPAPESIESAAEPDKSPIRHALAFVPLILVGAVNKYFTIYLPKWYPNGFDFSSIGLKEFGRLDISSAAAIWSVEIALVIGIITTILFDWRSVFAQLKEGLNEGIGGALLASMNTGAEYGFGGIIAALPGFHKLSSGISHTFTDPLVNGAVTTTALAGITGSASGGMGIALSAMSEQYLQAIQAYNIPPEVMHRVISMASGGMDTLPHNGAVITLLAVTGLTHRQSYRDIFAITLIKTAAVFAVIAIYSLTGLV 4R0C Chain:A ((327-440)) --------------------------------------------------MEVTMASMA-GYLVLMFFAAQFVAWFNYSQLGLLLAVKGAAWLGALTVPKVVLLLLFVVLTALINLMIGSASAKWSILAPVFIPMLMLLGISPEASQAAYRVGDS-STNIITPLMP-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4R0C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 291 -37937 -130.37 -348.04 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.46 3D Compatibility (PKB) : -130.37 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.46 QMean score : 0.256

(partial model without unconserved sides chains): PDB file : Tito_4R0C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R0C-query.scw PDB file : Tito_Scwrl_4R0C.pdb: