Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKHSFSRFFGLGEKEQEPEIAEHDTNKEEILEIPVNAIVPNRFQPRTIFSDEKIKELAMTIHTHGIIQPIVVRHTEEEGQYELIAGERRWRAVQSLEWEKIPAIIKDFSDTETASVALIENLQREELSSIEEAHAYARLLELHDLTQEALAQRLGKGQSTIANKLRLLKLPQPVQEAIMEKKITERHARALIPLKQPELQVTLLTEIIEKSLNVKQTEDRVVKMLEQGQRKPKPRRKAFSRDTRIAMNTIRQSLSMVEDSGVKLNTEEEEFEEYIQLTIRIPK 1VZ0 Chain:C ((23-207)) -----------------------------VVRLPLASIRPNPRQPRKRFAEESLKELADSIREKGLLQPLLVRPQGD--GYELVAGERRYRAALMAGLQEVPAVVKDLTDREALELALVENLQREDLSPVEEARGYQALLEM-GLTQEEVARRVGKARSTVANALRLLQLPPEALEALERGEITAGHARALLML-EPEDRLWGLKEILEKGLSVRQAE-----------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1VZ0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 603 -77402 -128.36 -418.39 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain C : 0.79 3D Compatibility (PKB) : -128.36 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.595

(partial model without unconserved sides chains): PDB file : Tito_1VZ0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VZ0-query.scw PDB file : Tito_Scwrl_1VZ0.pdb: