Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQNLRRAVFAGLVAGTLSEGAAATAGDGGFVEDSELQFLARTYYFNRDYRDSPNNAGRNRFKPRSERNGYREEATQGLRLQFASGYTPGSLGFGLDAHAMLGLQLDSGGGRTGTGNLPVGADGHPDHRYGKVGGALRLRHGETRLKYGQTTTSAPVFAASSNRTLAGMAYGLLLEDRSFDGLLLEGGRFTAASGPGESKVRGDISTVYGRLGAYPVRLDAVGFLGGQWQAT-ERLQLSLYASRFDDIWQQAYFGASHRQPLGGERALRVDLDAYRTRDSGQSRFGRIDTLTSSLALGYEHGPQRITLAYQRVHGEQPFDYMAFGDGRSSASMVLANSVGYSDFNGPGERSWQLRYDLDLGALGLPGLS-----LHALHARGRAGASASSAAESIYAGLYGRDGRHRENDLGFAYRVKAGPLAGLALRASQAWHRGNASYLDGDIDETRLVVDYSRSIW 4FMS Chain:B ((8-396)) ----------------------------GFLEDAKASLTARNFHLHRNFVG-----------------GKAEEWTQSFILDARSGFTQGSVGFGLDVLGLYSLKLD-------------------ADDFGRLAVAGKLRVSNSELKIGEWMPVLPILRSDDGRSLPQTFRGGQLSANEIAGLTLYAGQFRGNSPRNDASMQ-DMS-LFGRPAATSDRFD---FAGGEYRFNGERSLLGLWNAELKDIYRQQYLQLQHSQPL-GDWLLGANLGGFRGRDAGSARAGKLDNRTVSALFSARYGLHTLYLGLQKVSGDDGWMRV-----NGTSGGTLANDSYNASYDNPGERSWQLRYDFDFVGLGLPGLTFMTRYLHGDHVR-LAGVTDDGSEWG------------RESELG--YTLQSGAFKRLNVRWRNSSQRRDWGS-NTRFDENRLIVSYPLSLL

General information: TITO was launched using: RESULT: Template: 4FMS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -10106 for 2842 contacts (-3.6/contact) + 2D Compatibility (PS) -36366 + (NN) -7806 + (LL) 1220 1D Compatibility (HY) -20000 + (ID) 6250 Total energy: -79308.0 ( -27.91 by residue) QMean score : 0.242

(partial model without unconserved sides chains): PDB file : Tito_4FMS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FMS-query.scw PDB file : Tito_Scwrl_4FMS.pdb: