Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFLFTNGKVLWGAVIAAFILSIVFYPFLPTQMPIHYDVANSPDLTVNKLAGTVMLPVLMVVFAWARKINWQFVFAVYILLICHIVVLCLAL----------- 4RFX Chain:A ((432-531)) MVETLTERNLDLEEKVRELRETV--GDLEAMNEMNDELQENARETELELREQLDLAAARVREAEKRVEAAQETVADYQQTIKKYRELTAHLQDVNRELMSQQ

General information: TITO was launched using: RESULT: Template: 4RFX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 9 -1756 -195.11 -19.73 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -195.11 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.042

(partial model without unconserved sides chains): PDB file : Tito_4RFX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RFX-query.scw PDB file : Tito_Scwrl_4RFX.pdb: