Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEVIKSLTDHRSIRSYTDEPVAQEQLDQIIEAVQSAPSSINGQQVTVITVQDKERKKKISELAG-----------GQPWI---------------DQAPVFLLFCADFNRAKIALEDLHDFKMEITNGLESV-LVGAVDAGIALGTATAAAESLGLGTVPIGAVRGNPQELIELLELPKYVFPLSGLVIGHPADRSAKKPRLPQEAVNHQETYLNQDELTSHIQAYDEQMSEYMNKRTNGKETRNWSQSIASYYERLYYPHIREMLEKQGFKVEK 3GH8 Chain:A ((56-247)) --EFYELLNKRRSVRFISSEHVPMEVIENVIKAAGTAPSGAHTEPWTFVVVKDPDMKHKIREIIEEEEEINYMKRMGKRWVTDLKKLRTNWIKEYLDTAPVLILI----------FKQVHGFA---ANGKKKVHYYNEISVSIACGLLLAALQNAGLVTVTTTPLNCGPR-LRVLLGRPSHEKLLVLLPVGYPS-RDATVPDLKRKALD-------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3GH8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 655 -100063 -152.77 -606.44 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -152.77 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.634

(partial model without unconserved sides chains): PDB file : Tito_3GH8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GH8-query.scw PDB file : Tito_Scwrl_3GH8.pdb: