Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDITITLDRSEQADYIYQQIYQKLKKEILSRNLLPHSKVPSKRELAENLKVSVNSVNSAYQQLLAEGYLYAIERKGFFVEELDMFSAEEHPPFALPDDLKEIHIDQSDWISFSHMSSDTDHFPIKSWFRCEQKAASRSYRTLGDMSHPQGIYEVRAAITRLISLTRGVKCRPE-QMIIGAGTQVLMQLLTEL-LPKEAVYAMEEPGYRRMYQLLKNAGKQVKTIMLDEKGMSIAEITRQ-----------QPDVLVTTPSHQFPSGTIMPVSRRIQLLNWAAEEPRRYIIEDDYDSEFTYDVDSIPALQSLDRFQNVIYMGTFSKSLLPGLRISYMVLPPELLRAYKQRGYDLQTCS-SLTQLTLQEFIESGEYQKHIKKMKQHYKEKRERLITALEAEFSGEVTVK--GANAGLHFVTEFDTRRTEQDILSHAAGLQLEIFGMSRFNLKENKRQTGRPALIIGFARLKEEDIQEGVQRLFKAV--YGHKKIPVTGD 1VP4 Chain:A ((44-422)) -----------------------------------------------------------------------------------------------------------DAISFGGGVPDPETFPRKELAEIAKEIIEKEYHYTLQYSTTEGDPVLKQQILKLLERMYGITGLDEDNLIFTVGSQQALDLIGKLFLDDESYCVLDDPAYLGAINAFRQYLANFVVVPLEDDGMDLNVLERKLSEFDKNGKIKQVKFIYVVSNFHNPAGVTTSLEKRKALVE-IAEKYDLFIVEDDPYGALRYEGETVDPIFKIGGPERVVLLNTFSKVLAPGLRIGMVAGSKEFIRKIVQAKQSADLCSPAITHRLAARYLERYDLLEQLKPTIELYRRKRTVMLNALEEYFSDIPGVKWVKSEGGLFIWLTLPEGFDTWEMFEYAKRKKVFYVPGRVFKVYDEPSPSMR----LSFCLPPDEKIVEGIKRLREVVLEYGKEK------

General information: TITO was launched using: RESULT: Template: 1VP4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2064 -210113 -101.80 -582.03 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -101.80 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.515

(partial model without unconserved sides chains): PDB file : Tito_1VP4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VP4-query.scw PDB file : Tito_Scwrl_1VP4.pdb: