Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGRLVFNYRKAMREPKKIQQLTENYSLPFAVELIPAINYFIFVGLCFGFWYGVRMIFPHAFDNSYVIVIFGIPFFLTMLVTKIKPEGKNI-YIYFFDFAKYYFFIKLPQKKYCNDRKIDLSNEKQIEFRKLVKVVDYSNETKNAYEGNTQEFAVNKNGRRVGVLPNKKQFDSYAK-- 1YAV Chain:A ((11-144)) LLEA--TVGQFMIEADKVAHVQVGNNLEHALLVLTKTGY------------------------TAIPVL--------------DPSYRLHGLIGTNMIMNSIFGLERIEFEKLDQITVEEVMLTDI---PRLHINDPIMKGFGMVINNGFVCVENDEQVFEGIFTRRVVLKELNKHI

General information: TITO was launched using: RESULT: Template: 1YAV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 596 -77906 -130.71 -594.70 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -130.71 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.255

(partial model without unconserved sides chains): PDB file : Tito_1YAV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YAV-query.scw PDB file : Tito_Scwrl_1YAV.pdb: