Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTTVTDALYAGCEAVVKIAWLNGLWLLFTLLGGVLFGWAPSTAAMCAVIRKWLMGQKDVPIFSLFLDTYKKEFLKVNAIGLAFSALLLILSANYHYFSASTNWLSFAVTSCTLLAGLLYIIALMYVFPLYVHYQLPLRKYIPQALLFGAMRPLTTGCMLIGCGFVLYLLYTLPGLIPFYGPCLFGLVLMFFALRG-FQKTEAQHHQAG 4JQF Chain:A ((190-368)) AEALSNPGALDLPSLTSLLSEKAKEFLMENRVQSFYQQELEMVESLLSLANQ-----------------PVIHSTTSKAIHSIFKNAIQLLQEKGLVFQKDDG------------FDNLYYV---------TREDKDLHRKIHRIIQQDCQKPNHME---KGCHFLHILACARLSIRPGLSEAVLQQVLELLEDQSDIVSTMEHYYTAF

General information: TITO was launched using: RESULT: Template: 4JQF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 676 -120136 -177.72 -719.37 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -177.72 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.357

(partial model without unconserved sides chains): PDB file : Tito_4JQF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JQF-query.scw PDB file : Tito_Scwrl_4JQF.pdb: