Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNLIYNEWLKIFSRAGTWVMIGILGLTMVGFAFLANHFSAGESNSHWKQELQAQNAELKKEIKEDPSLKDGYKETITLNDYRIEHNIPSDTGYTVWSYVTDSANFTILTGLFTIIIAAGIVANEFNWGTIKLLMIRPLSRFQILMSKYITVLLFGLLLLLILFIGSTLLGLIFFGTGGETAANIHLIYKDGHVIEQNMMGHLATTYLSESVSALMVATMAFMLSAVFRNSSLAVGFSIFLLVAGTTATAFIAAKFDWAKYILFANVDLTQYVDGTPLIKGMTMTFSLVMLAIYFIIFLLLAFGIFMKRDIAN 2VOF Chain:B ((2-24)) ---------------------------------------------EEWAREIGAQLRRIADDLNAQYE-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2VOF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2 98 49.00 4.26 target 2D structure prediction score : 0.87 Monomeric hydrophicity matching model chain B : 0.38 3D Compatibility (PKB) : 49.00 2D Compatibility (Sec. Struct. Predict.) : 0.87 1D Compatibility (Hydrophobicity) : 0.38 QMean score : 0.866

(partial model without unconserved sides chains): PDB file : Tito_2VOF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VOF-query.scw PDB file : Tito_Scwrl_2VOF.pdb: