Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKIKKKKLFIPIIILVLTAFLALIGYISIIFLGHYVIDEKKLILHASSKIVDQNGDEVASLYTENREPVSINEIPKQVREAFIAVEDKRFYEHHGIDAKSVGRAVYRDILAGGKVEGGSTITQQLAKNIFLTHDKTFLRKTKEVIIAINLERDYSKDKLLEMYLNQLYFGHGVYGIQAASHYYFNKEVKDLTVSEGAVLAAIPKAPSTYSPILHPDKNKERRDTILGMMNDQGYIS----AKEAV---TAQGRTLGLHVKKQSETPWFDSYIDLVIKEAEDKYSISGEQLLQGGYTIKVPLDSKLQKTAYQVMKEGSYY--PGTDQNAEGSAVFINNKTGGVEAAIGGRDYTSKGYNRV-TAVRQPGSTFKPLAVYGPAMQEKKFKPYSLLKDELQSYGDYTPKNY-DSRYEGEVTMSDAITYSKNAPAVWT---LNEIGVETGKSYLKANGIDIPDEGL-ALALGG-LEKGVSPLQLAGAFHTFAANGTYTEPFFISSIIDEDGETIADHKEEGKRVFSKQTSWNMTRMLQQVVKKGTATS----------GTYHGDLAGKTGSTSYTGVSGATKDAWFAGYTPKITGAVWMGYDKTDQNHYLKAGSSYPTRLFKDILTQAGETGHVFTKPKNVKELESPIELKPVKTLTADYTFKAAGLFTIELKWDAQEDDRAVYRIYVNKDGEETLLDSVEGKGSYEIPYANLFSGASYKIVPYNTQTKREGEGTDYVQPKLFSS 2Y2J Chain:A ((5-393)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ISEITYSDGTVIAS--------------------------ISRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKF--YRDLAAKEIEN-----------GGYKITTTIDQKIHSAMQSAVADYGYLLDDGTGR-VEVGNVLMDNQTGAILGFVGGRNYQENQNNHAFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMTLGEALNYSWNIPAYWTYRMLRENGVDV-KGYMEKMGYEIPEYGIESLPMGGGIE--VTVAQHTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVLSSRVTTTFKSNLTSLNPTLANADWIGKTGTT------GQDENMWLMLSTPRLTLGGWIGH---DDNHSLSQQAGY---------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2Y2J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1819 -95307 -52.39 -276.25 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -52.39 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.346

(partial model without unconserved sides chains): PDB file : Tito_2Y2J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Y2J-query.scw PDB file : Tito_Scwrl_2Y2J.pdb: