Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKDKLRYAILKEIFEGN---TPLSENDIG-----VTEDQFDDAVNFLKREGYIIGVHYS-DDRPHLYKLGPELTEKGENYLKENGTWSKAYKTIKEIKDWIK 4EJO Chain:A ((21-98)) ----TLVMLVLSQLR-EPAYGYAL-VKSLADHGIPIEANTLYPLMRRLESQGLLASEWDNGGSKPR---KYYRTTDEGLRVLREVEA----------------

General information: TITO was launched using: RESULT: Template: 4EJO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 218 -31027 -142.33 -449.67 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -142.33 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_4EJO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EJO-query.scw PDB file : Tito_Scwrl_4EJO.pdb: