Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEANDQTQNIPAISFRSVRKSYKQDGQTYDVLQDVTGDIQQGAIVAVLGPSGSGKSTLLSMCNLMRTPDSGEVNIYGKEVREWNVNELRRTAALAFQSAPVLDGTVRDNLSLVQ-RLHQSQLYSPEKLASLAGLPPEL-------LDRSARDLSGGQRQRLSLARTLSNPSSILLLDEITSALDPVSALEIEELIKRQHQE--KKWTVMWVTHNMEQAKRIADTIWFMADGRLLEIAETDTFFSAPQHEAAKEFLKGGTR 2PMK Chain:A ((4-231)) ------------ITFRNIRFRYKPDSPV--ILDNINLSIKQGEVIGIVGRSGSGKSTLTKLIQRFYIPENGQVLIDGHDLALADPNWLRRQVGVVLQDNVLLNRSIIDNISLANPGMSVEKVIYAAKLAGAHDFISELREGYNTIVGEQGAGLSGGQRQRIAIARALVNNPKILIFDEATSALD----YESEHVIMRNMHKICKGRTVIIIAHRLSTVKN-ADRIIVMEKGKIVEQGKHKELLSEPE-------------

General information: TITO was launched using: RESULT: Template: 2PMK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1040 -129726 -124.74 -595.07 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -124.74 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.426

(partial model without unconserved sides chains): PDB file : Tito_2PMK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PMK-query.scw PDB file : Tito_Scwrl_2PMK.pdb: